bubbles in sheath

r_hicks@icrf.icnet.uk
Thu, 08 Sep 1994 16:16:23 BST

You could try degassing some buffer with a vaccuum, and then putting thedegassed
buffer into a bag/balloon inside your pressure vessel with the neck ofthe
bag/balloon tied to the outlet pipe of the sheath tank. This arrangementshould
prevent the sheath from saturating with gas, thereby preventingdepressurisation
bubbles. Alternatively you could try a mechanical (eg syringe) rather than an
air pump, this would probably be more expensive and intrusive(depending on what
machine you use) than the first option
Ray


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu