 |
 |
 |
 |
2. The bubbles are presumably air coming out of solution due to warming
up in the afternoon. You could try degassing your sheath fluid,
e.g. putting it under partial vacuum or heating it, before use.
3. My impression is that air bubbles have a very low side scatter to
forward scatter ratio, so could be gated out on that basis. But I'd
like to hear from anyone who really knows how air bubbles look to the
cytometer.
In message Tue, 06 Sep 1994 10:56:53 -0800,
"Betsy R. Robertson" <brrob@ims.alaska.edu> writes:
>
> Late-afternoon air bubbles in sheath fluid have been a problem lately.
> They can look very much like the bacteria I am analyzing. Does anyone
> have a source or a good design for a 2-4 liter sheath tank which will
> eliminate/minimize/ reduce(?) this problem?
>
> Betsy R. Robertson
> Institute of Marine Science
> University of Alaska Fairbanks
> Fairbanks, Alaska 99775-1080
> Phone (907)474-7709
> FAX (907)474-7204 e-mail: brrob@ims.alaska.edu
>
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
|
|
|
|