LDS-751
Dennis_Young@CIS.ucsd.edu
Fri, 12 Aug 1994 17:12:00 -0700
I have a users interested in using LDS-751 to try to discriminate cells digested
from ligaments. They want to gate out dead cells debris, but alot of the debris
overlaps with the cells. They are only using FITC as a second label and basicly
just need help in finding what concentrations and incubation times they should
start with. They haven't been getting good enough results with PI, and I thought
that using a PE label and FDA wouldn't be very sensative because fo the
fluorescein overlap.
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* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
* University of California, San Diego e-mail: djyoung@ucsd.edu *
* *
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
