autofluorescence
Mario Roederer (ROEDERER@Beadle.Stanford.EDU)
Thu, 24 Mar 1994 14:08:56 -0700 (PDT)
We have approached the problem of autofluorescence from a different tack
for a number of different applications: compensation. The fluorescence
in a PE channel for unstained cells is reasonably well correlated with
that in the FITC channel, especially for highly autofluorescent cells.
Thus, by using the PE fluorescence to compensate FITC, one can
essentially remove a majority of autofluorescence from the FITC channel.
This improves sensitivity considerably (10x?) for measuring FITC
fluorescences. References for doing this real-time (Alberti, Parks, and
Herzenberg) and in software (Roederer and Murphy) can be found in
Cytometry in 1986.
mr
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