Cy5
Dennis_Young@CIS.UCSD.EDU
21 Mar 94 11:16:00 -0800
We have very autofluorescent cells (so have stopped trying to use FITC) and
we're hoping to try to label nuclear biotin with a mouse anti-biotin antibody
that's Cy5 labelled. We've been trying to use strep-PE, but I'm not sure if
it's able to penitrate the nucleus. We have only Argon lasers, but one is
tunable on our FACStarPlus. Should I use 514 nM or just the regular 488 nM to
get the strongest signal? The emission should be around 580 nM?
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
