Re: fluorescent dyes
Harbor Branch Oceanographic Institution (harbolon@CLASS.ORG)
Wed, 16 Mar 1994 18:47:08 -0800 (PST)
This may sound kinda crazy, but I have been doing this awhile and it
seems to work. If you mix cells (viable) with trypan blue and excite at
488 nm, believe it or not, they emit in the red region (645). If you
look at these cells on a microscope with appropriate excitation, they
emit a bright red ring fluorescence. And, if the cells are dead, you
guessed it! They take up trypan and go from the red ring to full red
cell fluorescence. You can see the two different populations on a
histogram with the dead cells downfield from the live ones. I don't know
if this answers you question, but it is a neat trick to measure viability
without using P.I.
--Ross--
Ross E. Longley, Ph.D. * Harbor Branch Oceanographic Inst., Inc.
Immunology Group Leader * 5600 U.S. Highway #1, North
Div. Biomed. Marine Res. * Fort Pierce, FL 34946
Phone (407) 465-2400, 486
FAX (407) 465-1523
e-Mail harbolon@class.org
On 15 Mar 1994, Alice L. Givan wrote:
> Hello Flowers,
> Does anyone have any ideas about what my best options are for a fluorescent
> dye that is totally excluded by cells, that fluoresces in its free form, and
> that is excited at either 488 nm (or possibly in the UV).
> Thanks.
> Alice Givan
> NCCC Cell Analysis Lab
> Dartmouth Med School
> Lebanon, NH 03756
> USA
>
>
>
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