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© 1990-2002 J.Paul Robinson,
Purdue University BMS 631 –
LECTURE007.PPT
Basics of Flow Cytometry
•cells in suspension
•flow in single-file
through
•an illuminated volume
where they
•scatter light and emit
fluorescence
•that is collected,
filtered and
•converted to digital
values
•that are stored on a
computer
Fluidics
Optics
Electronics
[RFM]
