BMS 631 - LECTURE 1 Flow Cytometry: Theory J.Paul Robinson Professor of Immunopharmacology School of Veterinary Medicine, Purdue University

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Detectors

•Light must be converted from photons into volts to be measured •We must select the correct detector system according to how many photons we have available •In general, we use photodiodes for forward scatter and absorption and PMTs for fluorescence and side scatter