PPT Slide

Step 1: Cell Culture

Step 2: Cell Wash

Lab-Tek

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top view

side view

170 ?M coverslip

Step 3: Transfer to Lab-Tek plates

confocal microscope

oil immersion

objective

37o heated stage

stimulant/inhibitor added

Step 4: Addition of DCFH-DA, Indo-1, or HE

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