Superoxide: Utilizes hydroethidine the sodium borohydride reduced derivative of EB
Hydrogen Peroxide: DCFH-DA is freely permeable and enters the cell where cellular esterases hydrolyze the acetate moieties making a polar structure which remain in the cell. Oxidants (H2O2) oxidize the DCFH to fluorescent DCF
Glutathione: In human samples measured using 40 ?M monobromobimane which combines with GSH by means of glutathione-S-transferase. This reaction occurs within 10 minutes reaction time.
Nitric Oxide: DCFH-DA can also be used as an indicator for nitric oxide in a manner similar to H2O2
