Summary Document

This document is a summary of message from the Purdue University Cytometry Laboratories Cytometry Discussion list.

http://www.cyto.purdue.edu/hmarchiv/index.htm

Thanks again,
Joanna

• If you are working on human skin, then CD1a is a terrific marker for Langerhans cells. There are quite a few antibodies available. For the other cell types, I'm not aware of specific cell surface markers that are used in flow cytometry, although this may just be my ignorance. Several different anti-cytokeratin antibodies are used commonly in immunohistology for labeling keratinocytes, and these could work by flow if the cells are permeablized.
  
• I am not familiar with this either, but Langerhans: CD1a.
  
• I started to look at this a little while ago. These are papers that I thought may be useful…
  
• Immunofluorescent surface labelling, flow sorting and culturing of putative epidermal stem cells derived from small skin punch biopsies.
  M. M. van Rossum et al 2767 (2002) 109-117 Journal of Immunological Methods
  
• Prolonged MHC class II expression and CIITA transcription in human keratinocytes
  A Takagi et al 347(2006) 388-393 Biochemical and Biophysical Research Communications
  
• Marker succession during the development of keratinocytes from cultured human embryonic stem cells
  H Green et al 100 (2003) no. 26 15625-15630 PNAS
  
• Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence
  K Jensen et al 103 (August 2006) no 32 11958-11963 PNAS
  
• Immortalized keratinocyte lines derived from human embryonic stem cells
  S Iuchi et al 103 (2006) no. 6 1792-1797 PNAS
  
• Novel Functional Multiparameter Flow Cytometric Assay to Characterize Proliferation in Skin
  J. M. Mommers et al 42 (2000) 43-49 Cytometry (Communications in Clinical cytometry)
  
• Enrichment for murine keratinocyte stem cells based on cell surface phenotype
  H Tani et al 97 (2000) no. 20 10960-10965 PNAS
  
• The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells
  J G W Nijhof et al 133 (2006) 3027 –3037 Development