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Subject: Summary: Alternatives to CFSE staining for proliferation studies
Date sent: Thu, 24 Mar 2005 14:25:19 -0800
From: "Roger Burger" <rburger@miltenyibiotec.com>
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Thanks to all who replied to my question on CFSE
alternatives. Here is a
summary of the suggestions.
---------------------------------------------------------------------
From Mol Probes. Not as pretty as CFSE, but it
works with a red diode
laser.
CellTrace(tm) Far Red DDAO-SE
*special packaging*
Catalog Number
C34553
---------------------------------------------------------------
You could try SNARF from molecular probes. It
emits in the FL2 or FL3
channel.
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Ki-67 is just as good as CFSE.
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In my experience, with flow-based LPAs from hiv-
infected patients, CFSE
provided indirect evidence of toxicity (lower
proliferative activity in
CFSE-stained versus non-stained samples). I opted
for PKH26 from Sigma.
It runs on FL2. The main problem with PKH is the
price (much more
expensive). It also provides less precise peaks, but
use of Modfit
software for analysis compensated for this problem.
---------------------------------------------------------------------
PKH-26 or the other PKH dyes. These bind to lipids
but can be used for
the same purpose
---------------------------------------------------------------------
PKH 26 or PKH67 are excellent alternatives...they
stain the lipid
membranes in stead of the intracellular proteins. We
use them all the
time.
Roger A. Burger, Ph.D.
Technical Scientist
Miltenyi Biotec, Inc.
12740 Earhart Ave.
Auburn, CA 95602
530-887-5351
fax: 530-745-2851
