anti-IgG

I am in search of an unconjugated polyclonal F(ab')2 fragment of
anti-human IgG (H+L) antibody. I have tried Dako rabbit anti-human IgG
(Cat. No. A0423), Sigma Goat anti-human IgG (Cat. No. 027K4827) and
Rocklands Goat anti-human IgG (Cat. No. 709-1116), however these
antibodies either bind IgM or cross react with our mouse monoclonal
antibodies. Can anyone recommend an unconjugated polyclonal F(ab')2
fragment of anti-human IgG (H+L) that they have used successfully with
minimal species cross reactivity and that does not bind IgM.

 

Sheree Bailey

Senior Medical Scientist

Dept. of Immunology, Allergy and Arthritis

Flinders Medical Centre

Bedford Park SA 5042

AUSTRALIA