Hi Allessandro, We are using the "FOXP3 staining buffer set" (eBioscience) for Ki-67 staining. I don't know what the buffers contain, but they work very well for the purpose since they are designed to get the antibodies into the nucleus. Best regards, Samuel Lundin ________________________________ From: alessandro serra [mailto:serra@drfz.de] Sent: den 1 juli 2008 13:09 To: cyto-inbox Subject: ki-67 stain permeabilization? Dear All Can anybody suggest which is the best method to permeabilize cells in order to obtain the most reliable staining with Ki-67 Ab from BD? Thank you very much Dr. Alessandro Serra Cell Biology Group Deutsches Rheumaforschungszentrum (DRFZ) Berlin Charitéplatz 1 10117 Berlin Phone: +49-30-28460 765 Fax: +49-30-28460 603 Email: serra@drfz.de ___________________________________________ Samuel Lundin, Docent in Immunology, Dept. of Microbiology and Immunology and MIVAC - Mucosal Immunobiology and Vaccine Center, Institute of Biomedicine, Göteborg University, Box 435; 40530 Göteborg; Sweden Phone: +46-31-7866207Received on Wed Jul 2 14:38:00 2008
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