Hi Alessandro, We have had good results simply from 70% ethanol permeabilisation. I dimly recall that we also tried paraformaldehyde (1% then 0.1% Triton X-100 and got roughly similar results but the ethanol was more convenient). It may depend on whether you also want to detect surface antigens when the fix and perm may be more appropriate. Does the BD data sheet not have a recommendation? Good luck, Derek On 1/7/08 12:09, "alessandro serra" <serra@drfz.de> wrote: > Dear All > > Can anybody suggest which is the best method to permeabilize cells in order to > obtain the most reliable staining with Ki-67 Ab from BD? > Thank you very much > > Dr. Alessandro Serra > Cell Biology Group > Deutsches Rheumaforschungszentrum (DRFZ) Berlin > Charitéplatz 1 > 10117 Berlin > > Phone: +49-30-28460 765 > Fax: +49-30-28460 603 > Email: serra@drfz.de > > -- *************************************************************** Derek Davies, FACS Laboratory, London Research Institute, Cancer Research UK, 44 Lincolns Inn Fields, London, UK. Tel: (44) 20 7269 3394 FAX: (44) 20 7269 3479 Mobile: 07790 604112 e_mail: derek.davies@cancer.org.uk http://science.cancerresearchuk.org/sci/facs/ In tenebris lux ***************************************************************Received on Wed Jul 2 13:38:00 2008
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