Hello Sania, Did you examine by microscopy whether bacterial DNA (in the bacteria invading your cells) is not TUNEL-positive? It is possible that being degraded bacterial DNA may become a good primer to terminal transferase and undergoes labeling during the TUNEL reaction. Besides, microscopy is the only sure way to distinguish apoptosis from necrosis. Good luck! Zbigniew Darzynkiewicz, M.D., Ph.D. Professor of Pathology and Medicine Director, Brander Cancer Research Institute New York Medical College BSB, Room 438 Valhalla, N.Y. 10595 www.darzynkiewicz.com/zbigniew/ On 27/6/08 13:52, "Sānia Alves dos Santos" <saniasan@usp.br> wrote: Hi Flowers I'm working with hepatocyte culture, studying the frequency of apoptotic death in cells invaded by bacteria. Using the Tunel staining I've obtaining higher values in comparison to negative control cells (30%). Using Annexin staining the values are very low, about 1-5% of apoptotic cells. Otherwise the PI staining indicate necrosis in about 20% of cells. How can I interprete these results? Thanks in advance, Sania Alves dos Santos Laboratory of Bacteriology Faculty of Medicine University of Sao Paulo tel: (5511) 3061-7029 ************************************************************Received on Tue Jul 1 16:49:16 2008
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