Dear Flowers, I am a PhD student and doing flow cytometry on peripheral blood from healthy women. So far I have done quite a few samples and all of them are quite consistent in terms of results, for example CD3, CD4, CD8, CD14, CD66b expression. The attached FSC/SSC plot (FSC-SSC -1 file) looks very unusual to me as you can see most of the granulocytes are having low FSC and I am wondering if anyone have come across with this earlier and would appreciate any help/comments. First I thought that there might be some problem with the machine, so I did an experiment on my own blood (FSC-SSC -2 file) just to make sure that FACS machine is working fine and indeed I found the same reproducible result. I sincerely hope that someone here will be able to help me out in understanding what could be wrong here! Thank you all. Best wishes Abdul Khan PhD Student University of Glasgow This attachment - 'FSC-SSC -1 unstained 260608 .JPG' - 22.31 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/286724e98a43dc7207e01fca3ffcf8e3abac4964.JPG This attachment - 'FSC-SSC - 2 unstained 270608.JPG' - 22.07 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/0cc2a65dbcdb1e3b6a979e0fe90bbb1713b62d92.JPGReceived on Mon Jun 30 12:58:00 2008
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