RE: Particle sorting request

I'm not certain the size of the bacteria we used to sort but I don't
think they were much bigger than 200nm and we could pick them up fine
triggering off side scatter on a Vantage SE.  Your other option
depending on your instrument could be to trigger off of Alexa 568 and
then look at side scatter.   If this is something you want to do long
term and have a decent sized budget, you might want to talk to Cytopeia
in Seattle who custom make cytometers for analysis of cyanobacteria and
other tiny sea critters involved in carbon cycling.

 

Cheers,

Eric 

 

Eric O'Connor

Head of Flow Cytometry

MRC Clinical Sciences Centre

Imperial College Faculty of Medicine

Hammersmith Hospital Campus

DuCane Road, London  W12 0NN

 

Tel: +44(0) 208 383 8330

Mobile: +44(0) 781 575 7730

________________________________

From: Timothy Overton [mailto:two812@bham.ac.uk] 
Sent: 26 June 2008 15:17
To: cyto-inbox
Subject: Particle sorting request

 

Dear All,

 

I just has a request from someone working in my department which I think
is theoretically possible, but I wondered if anyone has tried anything
like it. They have nanoparticles coated in biotin, to which are attached
Streptavidin conjugated to Alexa 568. The size of the particles is
around 200 nm across. They asked whether flow cytometry could be used to
determine the proportion of particles with Strep-Alexa attached, and
then if they could be sorted. Has anyone done anything like this?

 

Thanks,

 

Tim

 

 

*****************************************

Dr Tim Overton

Lecturer in Biochemical Engineering

School of Chemical Engineering

The University of Birmingham 

Birmingham B15 2TT

t: +44 (0) 121 414 5306

e: t.w.overton@bham.ac.uk