Annexin x Tunel

   Hi Flowers

   I'm working with hepatocyte culture, studying the frequency of  
apoptotic death in cells invaded by bacteria. Using the Tunel staining	
I've obtaining higher values in comparison to negative control cells  
(30%). Using Annexin staining the values are very low, about 1-5% of  
apoptotic cells. Otherwise the PI staining indicate necrosis in about  
20% of cells. How can I interprete these results?

   Thanks in advance,

   Sania Alves dos Santos
Laboratory of Bacteriology
Faculty of Medicine
University of Sao Paulo
tel: (5511) 3061-7029