FW: Safety of serum samples on Flow Cytometry

Dear Renana,

You don't say if your samples can be inactivated in any way that would
kill the viruses without affecting your cytokine measurement.

You will need to talk to your Biological Safety Advisor or your local
equivalent to get clear guidelines. In UK, samples where the presence of
a Hazard group 3 pathogen such as HBV and HCV is confirmed would require
to be handled in a containment level 3 laboratory. You will need to
perform a Risk Assessment to identify the hazards that your procedure
involves and then put in places appropriate measures to avoid, or if not
possible, minimize operator exposure. I assume most of the people that
will be in contact with the samples will be already HepB vaccinated,
otherwise you might want to consider adding this to the list of
precautions.

 

Hope this help

 

Mara

 

Mara Rocchi BVM&S, PhD

Flow Cytometry Manager

Moredun Research Institute

Pentlands Science Park

Bush Loan, Penicuik

EH260PZ

Scotland, UK

 

 

Think before you print. Save paper and help the environment

 

________________________________

From: Renana Patoka [mailto:Renana@canfite.co.il] 
Sent: 25 June 2008 07:32
To: cyto-inbox
Subject: Safety of serum samples on Flow Cytometry

 

Dear fellows,

 

We are looking at HCV and HBV patients and would like to look at the
cytokines in the patients serum by Flow Cytometry. What special
precautions should we use? A general guideline on how serum samples
should be used on FACS will be appreciated also.

 

Thanks,

 

Renana Patoka


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