The issue of buildup of salt on FACSVantage Diva is related to relative humidity in sorter environment. When humidity is low and air is dry the salt buildup is amplified. When humidity levels are higher the problem becomes less apparent. David Hi Frank, We also saw this on our FACSVantageDiva a couple of years ago - also on the 70 micron nozzle at 30psi. It disappeared after a couple of weeks and has not reoccurred. Conjecture at the time was that there were some "issues" with the in-house PBS but we were not able to get any further than that. Another possibility was the nozzle attachment - we replaced several o-rings around that time because of seepage. The visible seepage didn't correlate with the salt build so it wasn't an obvious cause but I guess there might have been low level seepage that could have been contributing. Let us know if you work it out. Regards, Adrian Smith Centenary Institute, Sydney, Australia On 24/06/2008, at 10:52 PM, Glenn Paradis wrote: >Hi Frank, > >We saw the exact same phenomenon way back in the 1980's with our >FACStar Plus (10 PSI) and then later with our FACS Vantage with >Turbo Sort Option (30 PSI). A quick nozzle flush fixed the salt >buildup. Switching nozzles did not solve the problem. Our solution >was to move away from BD FACS Flow sheath fluid and go to NERL >Diluent 2. When we switched, we never had the salt buildup again. > >Good luck. > >Glenn Paradis >MIT Flow Cytometry Core Facility ><mailto:gap@mit.edu>gap@mit.edu > > > >On Jun 22, 2008, at 11:00 PM, WEHICytometry wrote: > >>In 31 years of flow cytometry I hadn't seen this before (although I >>don't get out much). Lately we experience this phenomenon on our >>FACSVantageSEDiVa where, in the course of a few hours, the nozzle >>grows a salt volcano around the stream (I've attached a picture). >> This does not affect the breakoff nor the sort streams nor even >>the acquired data until the volcano grows high enough to impinge on >>the first laser. It can also be removed easily by a wipe with a >>wet swab (although that's really inconvenient if the need arises >>during a sort). Note: we are talking about a 70 micron nozzle >>running PBS as sheath at 30 psi - nothing fancy. >> >>The question is: what causes it? We *see* no leaks outside the >>nozzle tip but I'm wondering if the orifice has eroded and that's >>causing seepage. Is there anyone who has seen this and/or has an >>idea of the cause? >> >>Frank Battye. >> >> | | << The Cytometry Laboratory >> \__/ <<<< The Walter & Eliza Hall Institute >>------!!<<<<<< 1G Royal Parade, Parkville >> /!!\ <<<< Victoria 3050, Australia >> o !! \ << ph: +61_3_9345 2540, fax: +61_3_9347 0852 >> >> >><DiVaNozzleVolcano.jpg> >> -- David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CPZN room 4310 Center for Regenerative Medicine and Technology Massachusetts General Hospital 185 Cambridge Street Boston,MA 02114 Tel (617)-726-1683 Fax (617)-724-3164 The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information.Received on Thu Jun 26 20:58:00 2008
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