Particle sorting request

From: Timothy Overton <two812@bham.ac.uk>
Date: Thu Jun 26 2008 - 10:17:24 EDT
Dear All,

 

I just has a request from someone working in my department which I think
is theoretically possible, but I wondered if anyone has tried anything
like it. They have nanoparticles coated in biotin, to which are attached
Streptavidin conjugated to Alexa 568. The size of the particles is
around 200 nm across. They asked whether flow cytometry could be used to
determine the proportion of particles with Strep-Alexa attached, and
then if they could be sorted. Has anyone done anything like this?

 

Thanks,

 

Tim

 

 

*****************************************

Dr Tim Overton

Lecturer in Biochemical Engineering

School of Chemical Engineering

The University of Birmingham 

Birmingham B15 2TT

t: +44 (0) 121 414 5306

e: t.w.overton@bham.ac.uk

 

 
Received on Thu Jun 26 20:18:00 2008

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