Dear Li Thanks for the input! The reason we are staining 100x10^6 cells is because we are trying to isolate them from human BM using anti-PE microbeads by the MACS technology. You are right, we can identify them using flow by gating 0.5x10^6 cells! Thanks Hadi On 6/25/08 3:46 PM, "chen li" <chen_li3@yahoo.com> wrote: > cytometry@flowcyt.cyto.purdue.edu Hadi Aslan PhD, DMD Skeletal Biotech Lab/Prof. Dan Gazit The Hebrew University of Jerusalem- Hadassah Medical Center PO Box 12272, Jerusalem 91120 Israel Tel: 972-2-6757625 Fax: 972-2-6757628 Mobile: 972-545-909208 Web site: http://gazitlab.huji.ac.ilReceived on Thu Jun 26 19:38:00 2008
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