Date: Tue Jun 24 2008 - 12:35:47 EDT
Dear flowers, I have just join a private company (I was previously working in the academic) and one of my topics is to install flow cytometry (not a problem for research use) by Quality Assurance (that is the problem) and I’m not familiar with QA. I have already found the Canadian Guideline for flow cytometry (2001) and the H42-A2 from NCCLS on the web and the daily/monthly QC of the instrument itself should not represent a big difficulty, but the validation of staining, antibodies panel, compensation, multicolor analysis, gating, reproducibility, what kind of calibrator should I choose…..remains unclear for me. Is anybody having a two (or more of course!) colors staining validation report (or validation plan)? It could be really helpful for me to adapt it and develop it for an 8 colors panel. Does more recent guidelines exist (FDA or CDC or else?) ? Thank you for your help. Best regards, Stéphanie Beq ******************************************** BEQ Stephanie Immunological Studies Manager cid:image001.gif@01C8B782.D5D90420 CYTHERIS S.A. Technopolis, 175 rue Jean-Jacques Rousseau 92130 ISSY-LES-MOULINEAUX, France <mailto:bassouline@cytheris.com> sbeq@cytheris.com www.cytheris.com Tél: 33(0)1 5888 3819 fax 33(0)1 4644 4446 STATEMENT OF CONFIDENTIALITY The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressees and may contain confidential or privileged information. If you are not the intended recipient, please notify "Cytheris" immediately at either (33)15888 3800 or at <mailto:info@cytheris.com> info@cytheris.com, and destroy all copies of this message and any attachments.
