Dear Frank, We experienced a similar problem. It can be avoided by spreading a thin layer of PTFE lubricant using a cotton swab. Best wishes, Jaroslav ********************************************************** I am pleased to announce publication of the first book on plant flow cytometry: http://www.wiley-vch.de/publish/en/books/bySubjectLS00/ISBN3-527-31487-3/?sID=69583a0477ef2333f7b43b719b83fac7 ********************************************************** Dr. Jaroslav Dolezel Laboratory of Molecular Cytogenetics and Cytometry Institute of Experimental Botany Sokolovska 6 CZ-77200 Olomouc Czech Republic Tel.: (+420) 585 205 852 Fax: (+420) 585 205 853 Email: dolezel@ueb.cas.cz Web: http://www.ueb.cas.cz/Olomouc1/LMCC/lmcc.html ********************************************************** Visit the first forum and blog on plant flow cytometry: FORUM: http://flowerdatabase.20.forumer.com/index.php BLOG: http://flowerdatabase.blogspot.com/ ********************************************************************** ----- Original Message ----- From: WEHICytometry To: Cytometry Mailing List Sent: Monday, June 23, 2008 5:00 AM Subject: DiVa nozzle salt volcano In 31 years of flow cytometry I hadn't seen this before (although I don't get out much). �Lately we experience this phenomenon on our FACSVantageSEDiVa where, in the course of a few hours, the nozzle grows a salt volcano around the stream (I've attached a picture). �This does not affect the breakoff nor the sort streams nor even the acquired data until the volcano grows high enough to impinge on the first laser. �It can also be removed easily by a wipe with a wet swab (although that's really inconvenient if the need arises during a sort). �Note: we are talking about a 70 micron nozzle running PBS as sheath at 30 psi - nothing fancy. � The question is: what causes it? �We *see* no leaks outside the nozzle tip but I'm wondering if the orifice has eroded and that's causing seepage. �Is there anyone who has seen this and/or has an idea of the cause? Frank Battye.� �� �|� � |� << The Cytometry Laboratory � �� \__/ <<<< The Walter & Eliza Hall Institute ------!!<<<<<< 1G Royal Parade, Parkville � �� /!!\ <<<< Victoria 3050, Australia � � o !! \� << ph: +61_3_9345 2540, fax: +61_3_9347 0852 ------------------------------------------------------------------------------ ------------------------------------------------------------------------------ In 31 years of flow cytometry I hadn't seen this before (although I don't get out much). Lately we experience this phenomenon on our FACSVantageSEDiVa where, in the course of a few hours, the nozzle grows a salt volcano around the stream (I've attached a picture). This does not affect the breakoff nor the sort streams nor even the acquired data until the volcano grows high enough to impinge on the first laser. It can also be removed easily by a wipe with a wet swab (although that's really inconvenient if the need arises during a sort). Note: we are talking about a 70 micron nozzle running PBS as sheath at 30 psi - nothing fancy. The question is: what causes it? We *see* no leaks outside the nozzle tip but I'm wondering if the orifice has eroded and that's causing seepage. Is there anyone who has seen this and/or has an idea of the cause? Frank Battye. | | << The Cytometry Laboratory \__/ <<<< The Walter & Eliza Hall Institute ------!!<<<<<< 1G Royal Parade, Parkville /!!\ <<<< Victoria 3050, Australia o !! \ << ph: +61_3_9345 2540, fax: +61_3_9347 0852 Received on Tue Jun 24 15:58:00 2008
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