Hi everyone, We have a few customers who bring samples that contain a lot of dead cells and red blood cells. The customers prefer not to lyse the rbcs or put their cells through a gradient to remove the dead cells and debris. They worry about decreasing the yield with the extra manipulation. We think that the rbcs and dead cells are sticking to our live cells and are sorted along with the population (giving us a lower purity). Depending on the downstream application, the contaminating rbcs/dead cells may negatively impact the results. Does anyone have suggestions of ways to increase the purity without changes in sample prep? We are having a difficult time excluding them with any kind of doublet gating using a MoFlo XDP. Any suggestions would be greatly appreciated! Thanks, Jacob R. Blair Research Specialist Cell & Immunobiology Core University of Missouri, Columbia One Hospital Drive, M324 MSB Columbia, MO 65212 Phone: (573) 884-7315 Fax: (573) 884-0665 Email: blairjr@health.missouri.edu <mailto:blairjr@health.missouri.edu> Web: www.biotech.missouri.edu/cic <http://www.biotech.missouri.edu/cic>Received on Tue Jun 24 13:18:00 2008
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