In 31 years of flow cytometry I hadn't seen this before (although I don't get out much). Lately we experience this phenomenon on our FACSVantageSEDiVa where, in the course of a few hours, the nozzle grows a salt volcano around the stream (I've attached a picture). This does not affect the breakoff nor the sort streams nor even the acquired data until the volcano grows high enough to impinge on the first laser. It can also be removed easily by a wipe with a wet swab (although that's really inconvenient if the need arises during a sort). Note: we are talking about a 70 micron nozzle running PBS as sheath at 30 psi - nothing fancy. The question is: what causes it? We *see* no leaks outside the nozzle tip but I'm wondering if the orifice has eroded and that's causing seepage. Is there anyone who has seen this and/or has an idea of the cause? Frank Battye. | | << The Cytometry Laboratory \__/ <<<< The Walter & Eliza Hall Institute ------!!<<<<<< 1G Royal Parade, Parkville /!!\ <<<< Victoria 3050, Australia o !! \ << ph: +61_3_9345 2540, fax: +61_3_9347 0852 Received on Mon Jun 23 15:58:30 2008
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST