washing nylon mesh

Hi
I am Amine, i am mesuring plant DNA content.
If possible i want to  know how to wash reliably my nylon mesh  without having dyes particuls, in order to reuse them.

Thanks 

Meddah Mohamed Amine
Laboratory of plant improving
Universite of Oran; ALGERIA.

--- En date de : Ven 20.6.08, Miller, Eric <Eric.Miller@crl.com> a écrit :
De: Miller, Eric <Eric.Miller@crl.com>
Objet: DNA content of bacilli
À: "Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu>
Date: Vendredi 20 Juin 2008, 12h15



 
Looking for some 
help here. Although I have a lot of experience in running DNA content on 
mammalian cells I have been asked to look into staining bacilli. The samples I 
will recieve will have differing DNA content, which I need to use to put a 
number to each cell type. The method suggested was with Hoecht 33342, but 
we do not have a UV laser. The methods I have cobbled together are 1) Fix and 
stain with PI, or 2) Stain with syto-9 (part of Invitrogen's Live/Dead 
discrimination kit. I just wondered if anyone did this sort of thing routinely, 
and if they had a protocol I might try.
Thanking in advance 
for your input,
Eric
 
Eric Miller
Senior Flow Cytometry 
Analyst
Charles River laboratories
Tranent
East Lothian
EH33 5NJ
Tel 01875614545
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