Re: Handling BSL2 flow samples

From: Steve Perfetto <perfetto@mail.nih.gov>
Date: Fri Jun 13 2008 - 19:34:03 EDT
Kathy,

Infectious cell sorting is certainly a subject of much discussion and the
following web site is a good start to understand some of the precautions
necessary in handling these sorts. Please note these are now standards,
which we hope to continue to develop as new technology and strategies become
available. In the near future, this site will publish the biosafety
lectures, which were presented at the ISAC conference.

http://www.isac-net.org/content/view/660/46/

SP


On 6/12/08 1:03 PM, "Schreiber, Kathy L." <schreiberkl@health.missouri.edu>
wrote:

> Hi,
>  
> Our flow cytometry facility will begin running human (fixed) samples on our
> analyzers soon and would like to know what special precautions should be taken
> for these and other BSL 2 samples. In particular, we know we will have
> HIV-infected samples, but we do not know what other pathogens we might be
> working with in the future. We have 3 analyzers in a separate room with a sink
> and limited access. Can anyone provide some general guidelines on how the
> samples should be handled and discarded? What sort of disinfection must be
> done for the analyzers and the surrounding benchtop?
>  
> Thanks in advance for any advice!
>  
> Kathy L. Schreiber, PhD
> Senior Research Specialist
> Cell & Immunobiology Core
> University of Missouri, Columbia
> One Hospital Drive, M324 MSB
> Columbia, MO 65212
> Phone: (573) 882-3626
> Fax: (573) 884-0665
> Email: schreiberkl@missouri.edu <blocked::">mailto:schreiberkl@missouri.edu>
> <mailto:schreiberkl@missouri.edu>
> Web: www.biotech.missouri.edu/cic
> <blocked::">http://www.biotech.missouri.edu/cic>
> <http://www.biotech.missouri.edu/cic>
> 


-- 

Stephen P. Perfetto, MS.,MT. (ASCP)
Manager, Core Flow Cytometry Facility
Vaccine Research Center, NIH
Building 40
40 Convent Dr., Room 5507
Bethesda, MD 20892-3015

email: sperfetto@nih.gov
Phone: (301) 594-8659

Disclaimer:

The information in this e-mail and any of its attachments is confidential
and may contain sensitive information.  It should not be used by anyone who
is not the original intended recipient.  If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices.  The National Institute of Allergy and Infectious Diseases
(NIAID)  shall not accept liability for any statement made that are the
senderıs own and not expressly made on behalf of the NIAID by one of its
representatives.







image.jpg
Received on Mon Jun 16 13:18:00 2008

This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST