Hi Rudjer, The ancient article cited below should be helpful. Different phylum, but sea squirts have a primitive heart beating a liquid around containing cells that one might describe as hemolymphs. The key to successful isolation and characterization of the cells described in the paper was that cell subsets were identified by autofluorescence under UV excitation. There was a hint the cell subsets identified by microscopy may be differentiated by light scatter and autofluorescence at visible wavelengths, but UV provided resolved populations. Cell cycle analysis should definitely be possible but I would recommend you initially start with DNA content relative to trout or chicken RBCs levels first - different cell subsets may have different levels of DNA. good luck, TomD, writing from home..... 28) Oltz EM; Pollack S; Delohery T; Smith MJ; Ojika M; Lee S; Kustin K; Nakanishi K Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. Department of Chemistry, Columbia University, New York, NY 10027. Experientia 1989 Feb 15;45(2):186-90 On Jun 4, 2008, at 7:31 AM, Rudjer Novak wrote: > dear all, > > im interested in flow cytometric analysis of mussel haemolymph cells. > The experiments will be a collaboration with colleagues in a marine > biology lab and so im looking into the area... > > Can anybody reccomend a good place to start? > What number of haemolymph cells can you extract from a single mussel, > what types of haemolymph cells are there, can they be easily > discriminated (FS vs SS?), are there any specific protocols, tricks > regarding work with haemolymph etc. > For starters, we will be looking into cell cycle variations in > different > populations (which sounds pretty simple...is it?). > > thanks, > rudjer > > ________________________ > > Rudjer Novak, dipl. ing. > Farmaceutsko-biokemijski fakultet > Zavod za biokemiju i molekularnu biologiju > A, Kovacica 1 > 10000 Zagreb > tel: 095 804 3102 > ________________________ > > Rudjer Novak, B.Sc. > University of Zagreb > Faculty of Pharmacy and Biochemistry > A. Kovacica 1, Zagreb > Croatia >Received on Thu Jun 5 13:18:00 2008
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