Dear Rudjer, I assume from the context of your message that you mean, what is the structural difference between IgG isotypes, rather than allotype? A comparison of the amino acid differences between different rat, mouse, and human sequences can be obtained from my website as http://www.path.cam.ac.uk/~mrc7/lecturenotes/handout1a.pdf The table is adapted from a published review of mine Clark, M (1997), Chemical Immunology 65, 88-110 Antibody Engineering IgG Effector Mechanisms Which can be downloaded from my website as a preprint (sorry I don't have reprints available electronically) http://www.path.cam.ac.uk/~mrc7/publications/preprints/PP1997CI65_88.pdf This review references our earlier work Bruggemann et al, J. Immunol (1989) 142: 3145-3150 which covers the structural and some of the functional differences of the rat subclasses. Cheers, Mike On May 27 2008, Rudjer Novak wrote: >dear flowers, > >I use a primary (rat) IgG2a antibody and need a secondary, but I can't >find an IgG2a specific one. The best I could find was a polyclonal anti >rat IgG Ab and a H+L specific anti rat IgG Ab (which sound the same to >me?). Is that gonna work? Would you prefer any of the mentioned >secondaries? What is the structural difference between IgG allotypes? > >thanks for your time! >rudjer > -- M.R.Clark, PhD Reader in Therapeutic and Molecular Immunology University of Cambridge, Department of Pathology Tennis Court Road, Cambridge CB2 1QP Tel +44 1223 333705 Fax +44 1223 333875Received on Tue Jun 3 11:38:00 2008
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