Re: Amine-reactive dyes and impaired functionality

Suzanne,

Early studies with the amine reactive dyes and measurement of cytokine
function (secreted cytokines) showed no differences between treated and non
treated samples. There are now many experiments using ICS and other
procedures (multimers and proliferation assays) showing no issues with the
use of amine reactive dyes.

Hope this helps.

SP


On 5/23/08 9:43 PM, "Suzanne Mertens" <saguila@emory.edu> wrote:

> Dear Colleagues,
> I have a Core client who is interested in using amine-reactive Alexa430 as a
> viability
> dye during a cell sort. Should I be  concerned about impaired functionality
> for the
> sorted cells previously stained with such a reagent?. Is there any data out
> there for
> functional performance of cells stained with an amine reative dye, sorted, and
> then
> cultured for cytokine assays?
> 
> Suzanne
> 
> Sent from my Verizon Wireless BlackBerry
> 

-- 

Stephen P. Perfetto, MS.,MT. (ASCP)
Manager, Core Flow Cytometry Facility
Vaccine Research Center, NIH
Building 40
40 Convent Dr., Room 5507
Bethesda, MD 20892-3015

email: sperfetto@nih.gov
Phone: (301) 594-8659

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