Re: Amine-reactive dyes and impaired functionality

We have not looked at the effect of amine-reactive dyes on long-term  
viability.  However...	why use the amine-reactive dyes if you want to	
culture long-term?  The amine reactive dyes are good because they  
withstand fixation.  If you're not going to fix, you should use PI,  
the king of all viability dyes!

mr

On May 23, 2008, at 9:43 PM, Suzanne Mertens wrote:

> Dear Colleagues,
> I have a Core client who is interested in using amine-reactive  
> Alexa430 as a viability
> dye during a cell sort. Should I be  concerned about impaired  
> functionality for the
> sorted cells previously stained with such a reagent?. Is there any  
> data out there for
> functional performance of cells stained with an amine reative dye,  
> sorted, and then
> cultured for cytokine assays?
>
> Suzanne
>
> Sent from my Verizon Wireless BlackBerry