Hello everyone. Is anyone here familiar with the requirements for successful genomic DNA isolation and methylation analysis of different genes? I would like to perform this analysis on cells that can however only be isolated after FACS sorting of cells that have previously been fixed and permeabilized for detection of intracellular antigens. What I am mainly concerned with is whether fixation and permeabilization might denature the DNA in these cells, and how this would affect any subsequent analysis including successful DNA isolation etc. Google and pubmed searches have been unsuccessful so far, so I am grateful for any suggestions. Thanks a lot, anja _______________________________________________ Anja Scholzen PhD Student Monash University Department of Immunology Vaccine Development and Infectious Diseases Laboratory Commercial Road, Prahran 3181 VIC, Australia Office: 0061 3 9903 0716 Lab.: 0061 3 9903 0223 Fax: 0061 3 9903 0038Received on Fri May 23 11:58:00 2008
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