Dear all! We are considering conjugating a monoclonal Ab to Alexa Fluor 647, 660 or 680 to be used by flow cytometry. Would you be able to tell me which one has the largest Strokes Shifts and would be the brightest to be use by flow cytometry, please? Also, which one would be excited better with a 633 nm He/Ne laser? We are planing doing a multicolour experiment using the following flourochromes: FITC, PE, PE-Texas Red plus one of the above mentioned Alexa Fluor. Also, do you know which filter would I need to detect the Alexa Fluorochorme you would recommend for our experiments on a BD FacsVantage? Thanks a lot for your comments! Virginia -- Dr Virginia Portillo The University of Manchester Manchester Interdisciplinary Biocentre 131 Princess Street Manchester M1 7DN Tel: 0161 306 5820 Fax: 0161 236 0409Received on Tue May 20 12:58:00 2008
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