Jenny, I have had a Guava EasyCyte Mini since December and love it!! It is the ideal instrument for my lab because we have periods of time where we do lots of analysis and other periods of time where we may not use it for a couple of weeks. We have not had any issues with not running it on a daily basis. Due to the fact that it prefers a low cell concentration in your final volume, we typically label 10 uL of human or mouse blood and get excellent results. This is opposed to other instrument (Coulter FC500) where we used to have to label 100 uL of blood. So essentially less sample and less reagent = money saved. We use the cell counting / viability (ViaCount) routinely and have found that it works well. We run unlysed blood through it and other than running an extra cleansing cycle have not had any blockage problems. The other good news is that the flow cell is really easy to remove (via a small port on the top), so if you have to clean it, this can be done very quickly. Overall I think it is a great machine from my limited experience over the past few months. If you would like to discuss it more over the phone, let me know. Good Luck!!! Brian -- Brian K. McFarlin, PhD Assistant Professor Exercise Physiology / Nutrition University of Houston Department of Health and Human Performance 3855 Holman St. Houston, Texas 77204-6015 (713) 743-9929 phone (713) 743-9860 fax On 5/7/08 1:29 AM, "Bryant, Jenny (NSW)" <JBryant@arcbs.redcross.org.au> wrote: > Hi, > > We are considering purchasing a Guava instrument, initially for cell count/% > viability/% apoptosis measure of all patient and donor samples referred to our > Tissue Typing lab for (CDC & Flow) crossmatching and cellular studies. > > I would appreciate any feedback from Guava users on ease of use, maintenance, > flow cell blockages, which applications you have tried and their success - > all info is helpful and confidential. > > I look forward to hearing from you, > Regards > Jenny > > Jenny Bryant > Senior Scientist > Flow Cytometry Laboratory > Tissue Typing Services > ARCBS - Endeavour > 153 Clarence St > Sydney NSW 2000 > Ph 61 2 9229 4366 > Fax 61 2 9229 4487 > > Don't ignore the need for blood > S For general information call 13 14 95 or visit www.donateblood.com.au > <file://www.donateblood.com.au> > >Received on Thu May 8 14:58:00 2008
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