Re: ELISA for cytokines

Dear Alamelu,

For instance, QuantiFERON made by Cellestis (www.cellestis.com) 
measures IFN-g as soon as after overnight incubation in the method 
approved by the FDA (www.fda.gov/cdrh/pdf/P010033b.pdf) and other 
regulators. You will get a lot of information by searching 
"tuberculosis+interferon" or else.

Regards.

Vladislav Rozenkov


-----Original Message-----
From: RAJA ALAMELU <alameluraja@yahoo.com>
To: cyto-inbox
Sent: Wed, 7 May 2008 4:00 pm
Subject: ELISA for cytokines



Dear Flowers,

 

This is a flow unrelated question. But I am sure the flow community 
will be readily forthcoming with their responses, as always.

 

What is the optimal time for measuring the following cytokines, in 
human PBMC culture supernatants, stimulated with mycobacterial antigens 
by ELISA?

 

1. IFN-g

2. IL-4

3. IL-10

4.TGF-b 

 

Thanking you in advance,

 

Alamelu Raja,

Tuberculosis Research Centre (ICMR),

Mayor V. R. Ramanathan Road,

Chetput,

Chennai - 600 031,

INDIA.

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