hello, not directly an answer concerning the dectin-1 antibody, but you can also use CD11c (in combination with CD11b and autofluo): http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_u ids=15382026 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_u ids=12960339 concerning the FITC signal added to the autofluorescence: yes, that should work - but I'd only do it in lack of other options. Regards, Pieter Natalia Muñoz Wolf wrote: > Hi all! > I´m currently working in a model of acute pneumonia in mouse. I need > to improve the FACS analysis of the BAL and single cell suspension we > prepare from lungs. I´m having some trouble identifying alveolar > macrophages because we´re using F480 but this marker is poorly > expressed in lungs. > I found a reference at the Journal of Immunology in wich they > demonstrate that DECTIN 1 (also knokw as beta glucan receptor) is > highly expressed by different types of macrophages including alveolar > macs, but also in other cell types. (See reference at: /J of Immunol/, > Taylor RP /et al/, 2002, 169: 3876-3882 or > http://www.jimmunol.org/cgi/content/full/169/7/3876). > FITC and PE conjugated anti-Dectin 1 markers are commercially > available for flow cytometry. I wonder if I should use it because this > marker is also expressed by neutrophils, some subsets of DCs and T cells. > Have you ever tryed it? Can I use the FITC conjugated marker besides > the high autofluorescence of the macrophages? > > Thank you in advance! > > Natalia > > > > -- > Natalia Muñoz Wolf > Laboratory for Vaccine Research, > Department of Biotechnology, > Instituto de Higiene, > Facultad de Medicina > Avda. A. Navarro 3051, > Montevideo, CP 11600 > Uruguay > Tel.: + 5982 487 12 88 (ext. 1121) > Fax: + 5982 487 30 73 > email: nataliam@higiene.edu.uy <mailto:nataliam@higiene.edu.uy> -- Pieter Bogaert Lab Molecular Signaling and Cell Death Ghent University & VIB Fiers-Schell-Van Montagu' building Technologiepark 927 B-9052 Ghent (Zwijnaarde), Belgium Tel : +32-9-33 13 762 e-mail : pieter.bogaert@dmbr.UGent.beReceived on Wed May 7 18:38:00 2008
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