Sperm chromatin decondensation

I am looking at DNA staining of human sperm by flow and have seen completely
different propidium iodide staining patterns with two different
decondensation protocols.

Any advice about optimizing sperm chromatin decondensation and denaturation
would be greatly appreciated; especially, in the context of flow FISH or
FISH in suspension.

Thanks, 
Annie Bang.

		MOUNT SINAI SERVICES
		Annie Bang
		Flow Cytometry Centre
		Pathology and Laboratory Medicine
		Mount Sinai Hospital
		Room 6-209
		600 University Avenue
		Toronto, Canada  M5G 1X5
		Phone (1) (416) 586 4800 ext 6530
		Fax	(1) (416) 586 8628
 E-mail  abang@mtsinai.on.ca <mailto:kpritzker@mountsinaiservices.com> 

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