RE: green laser suitability

Hi Ralph...

The 532 nm laser will excite all but the longest of the red FPs (like mPlum) reasonably
well, but is particularly good for the shorter wavelength red proteins, like mOrange,
DsRed and dTomato.  We get better excitation of the longer red FPs (mStrawberry, mCherry,
mPlum, mKate and Katushka) with 561 and 580 nm sources.

532 nm lasers suitable for flow are available at power levels at least up to 150 mW. In
our hands, fluorescent protein s/n sensitivity does increase with higher power levels -
but our 50 mW 532 nm module has certainly been adequate for most detection tasks.  561 nm
lasers are now available up to 100 mW, and our 580 nm laser (MPBC, Canada) peaks at over
200 mW, although we usually run it at 50 mW on our LSR II, and never more than 100 mW.	

Take care,

Bill Telford
NCI-NIH

-----Original Message-----
From: Rossi Ralph [mailto:ralph.rossi@petermac.org]
Sent: Tue 4/29/2008 2:20 AM
To: cyto-inbox
Subject: green laser suitability
Dear all,

	does anyone have any recommendations/experience using ,green, 532nm lasers for
excitation of the various "fruit" (RFP variants: cherry ,tomato, etc) report proteins.
	The maximum power available( that I can find ) for one of these lasers is 50 mw ,
I would think this is adequate, is it  ?

thanks
				ralph

Regards 
Ralph Rossi
Flow Facility Manager
Peter MacCallum Cancer Centre
Melbourne , Australia
Email: ralph.rossi@petermac.org
Ph 96563747 , 96561955



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