Re: green laser suitability

The Coherent Vardi line has powers up to 18W - more than you'll ever  
need. See
http://www.cohr.com/Lasers/index.cfm?fuseaction=show.page&id=873&loc=834

We have a 150 mW 532 nm laser on our LSR and it works quite well with  
mCherry, mTomato, mRFP. It is also much better than the blue (480ish)  
lasers at exciting PE and its tandem conjugates (PE-TR, PE-Cy7, etc.)

Marty Bigos, Director
Gladstone Flow Core

mail:
J. David Gladstone Institutes
1650 Owens Street
San Frnacisco CA 94158

Phones:
(office) 415-734-4821
(mobile) 415-845-8450

fax:
415-355-0855



On Apr 28, 2008, at 11:20 PM, Rossi Ralph wrote:

> Dear all,
>
>	  does anyone have any recommendations/experience  
> using ,green, 532nm lasers for excitation of the various  
> "fruit" (RFP variants: cherry ,tomato, etc) report proteins.
>
>	  The maximum power available( that I can find ) for one of  
> these lasers is 50 mw , I would think this is adequate, is it  ?
>
> thanks
>				  ralph
>
> Regards
> Ralph Rossi
> Flow Facility Manager
> Peter MacCallum Cancer Centre
> Melbourne , Australia
> Email: ralph.rossi@petermac.org
> Ph 96563747 , 96561955
>
> This email (including any attachments) may contain
> confidential and/or legally privileged information and is
> intended only to be read or used by the addressee. If you
> are not the intended addressee, any use, distribution,
> disclosure or copying of this email is strictly
> prohibited.
> Confidentiality and legal privilege attached to this email
> (including any attachments) are not waived or lost by
> reason of its mistaken delivery to you.
> If you have received this email in error, please delete it
> and notify us immediately by telephone or email. Peter
> MacCallum Cancer Centre provides no guarantee that this
> transmission is free of virus or that it has not been
> intercepted or altered and will not be liable for any delay
> in its receipt.
>