Dear Flowers I have a problem with FacsAria When I sort stem cells from KASUMI cells linee (Acute MyeloId Leukaemia), I lost the cells. My guess is that the cells are being lost due to stickiness or cell death. Looking at FSC vs. SSC and gating on the viable cells post sort only 10% of the events fall in what I would consider debris or dead cells. The cells are spin down and then counted. There are essentially no dead cells visible in these counts. Last time I sorted 3.200.000 cells and I have count only 11.000!! It's impossible!! I don't have the same problem with other cells type and I use always the same parameters ( PSI, Drop delay and Frequency) Can you help me? Any insights will be greatly appreciated. Ciao Simona -- Simona Ronzoni Imaging Centre Consortium for Genomic Technologies c/o IFOM-IEO Campus for Oncogenomics via Adamello 16 20139 Milan Italy Phone: ++39-0294375087/0294375038 email: simona.ronzoni@ifom-ieo-campus.it http://www.ifom-ieo-campus.it/RESEARCH/Imaging/ http://www.ifom-ieo-campus.itReceived on Tue Apr 29 14:18:00 2008
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