Dear all, One groups wants to separate Lolium nuclei from the organelles... This project is really challenging for me and I'll need some help. The main target is separating mitochondria (urgent application) and the second target is sorting chromosomes. Lolium has 7 chromosomes in the haploid set. As I told you I'm a little bit out of the Botany field now and I would like to come back... Do you have some protocols, procedures, papers, information, tips and tricks... that could help me? For analysis I think I shouldn't have big problems because of the equipments that I've in the lab, but for sorting I could be limited because I've a FACSAria and it has a 70 and a 100 um nozzles. Nevertheless, probably the higher restriction for chromosome sorting it could be that instead of a UV laser the Aria has a violet laser... Any help will be very welcome. Kind regards, Alfonso ------------------------------ Dr. Alfonso Blanco Fernández Flow Cytometry Core Facilities UCD - Conway Institute of Biomolecular & Biomedical Research University College Dublin Belfield, Dublin 4 IRELAND T: 00353(0)1 716 6836/6947Received on Fri Apr 18 13:58:00 2008
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