Dear Sean You may wish to try to CyGEL. This thermoreversible hydrogel will permit live-cell imaging of non-adherent cells for several hours. You can find more information at our website if this is of interest. Best regards Roy Edward Biostatus Ltd TEL: 01509 558163; roy@biostatus.com <mailto:roy@biostatus.com> ; www.biostatus.com <http://www.biostatus.com/> Co. #: 3079239. Regd. in England & Wales. Regd. office: 56 Charnwood Road, Shepshed, LE12 9NP, UK. ________________________________ From: Sean A Burke [mailto:sab541@nyu.edu] Sent: Fri 04/04/2008 17:15 To: cyto-inbox Subject: Adhering non adherents The discussion of removing adherent cells got me thinking about a problem I will have to solve in the near future. Although it is not flow related I was hoping for some input. I am looking to get Jurkat cells to adhere to a cover slip in order to do some time-lapse live- cell imaging which will involve perfusing the chamber they are in and possibly moving the cells. Does anyone have any experience with a way immobilize by adherence or any other medthod? Sincerely, Sean Burke Department of Basic Sciences New York University Dental Center 345 E 24th Street, S-921 New York, NY 10010 Phone: (212)-998-9276 >Received on Mon Apr 7 14:58:00 2008
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