I might suggest coating your chamberslides or Mattek dishes or whatever coverslip bottomed vessel you choose with one of the following: 1. Poly-lysine (d or l) 2. collagen 3. fibronectin or one of many adhesion molecules that may or may not cause some cell signaling changes in your cells, ie. madcam, vcam, icam. 4. Celltak from BD if one of the above doesn't "work" for your experiment. 5. Cygel from Biostatus is another option as well. Cheers, -- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 332 North Lauderdale St., E7061 Memphis, TN 38105-2794 (901) 495-2536 samuel.connell@stjude.org ________________________________________ From: Sean A Burke [sab541@nyu.edu] Sent: Thursday, April 03, 2008 1:30 PM To: cyto-inbox Subject: Adhering non adherents. . The discussion of removing adherent cells got me thinking about a problem I will have to solve in the near future. Although it is not flow related I was hoping for some input. I am looking to get Jurkat cells to adhere to a cover slip in order to do some time-lapse live- cell imaging which will involve perfusing the chamber they are in and possibly moving the cells. Does anyone have any experience with a way immobilize by adherence or any other medthod? Sincerely, Sean Burke Department of Basic Sciences New York University Dental Center 345 E 24th Street, S-921 New York, NY 10010 Phone: (212)-998-9276 >Received on Mon Apr 7 13:18:00 2008
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