Date: Thu Mar 27 2008 - 18:32:26 EDT
Cytometry Discussion group: I am attempting to determine by flow the number of nucleated red blood cells in cord blood by following the procedure of Tsuji T et al., Cytometry 37:291-301, 1999. Briefly, the protocol uses EDTA-treated PB with anti-CD45-FITC and PI (threshold FSC 52, FACSCalibur) with an acid hypotonic lysis followed by a neutral hypertonic alkaline reagent. I am using a BD Canto II with CPD-treated CB and we are following this protocol but have been unable to see any nRBC in cord blood. I appreciate any suggestions. Nicholas J. Greco, Ph.D. Director of Laboratory Operations Cleveland Cord Blood Center Suite 150 25001 Emery Road Cleveland, OH 44128-0804 216-896-0384 (office) 216-896-0320 (fax) 440-488-9205 (cell)
