Dear All Some of my colleagues claim that in order to be able to see human IL-10 by FACS, fixation of the cells should be performed in buffer without BSA. But that this is important only in this step and then BSA can be back in subsequent washes, permeabilization and staining. I have not run a comparative experiment yet. But, has anybody experienced any or similar phoenomena of interference in the staining by the protein in the buffer? I hope I am not covering myself in ridicule with this post, but what I heard sounded too strange to me. Dr. Alessandro Serra Cell Biology Group Deutsches Rheumaforschungszentrum (DRFZ) Berlin Charitéplatz 1 10117 Berlin Phone: +49-30-28460 765 Fax: +49-30-28460 603 Email: serra@drfz.deReceived on Wed Mar 19 15:38:00 2008
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