hi all i have FACS CALIBURE FLOWcutometer without sorter, i need to use this devise in stem cell quantitative to identifing the percentage of stem cell in my samples( whool blood and bon marrow ) pls i need acomplete prtocool for this application starting from separation of mononuclear cells to preparing to intoducing to flow? I have CD 34+ PE labelled AC 133 pure CD133 CALIBRITE BEADS 2 COLORS FACS LYSING SOLUTION ISOTYPE CONTROOL ANTI MOUSE IgG Isotype control Mouse Ig G1 PE hich can i use for this applcation: send aprotocol( complete) thanks ahmed áÇÅáå ÅáÇ Çááå ãÍãÏ ÑÓæá Çááå Ahmed EL-wakeel Flowcytometry Applications Specialist Stem cell Research Center Mobil: 0101046053 _________________________________________________________________ Connect to the next generation of MSN Messenger http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtaglineReceived on Tue Mar 18 14:38:00 2008
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