A Non-Flow question

Hello flow-ers,
A non flow question, and I hope some of you might have a lot of experience and tips for me. I am thinking of using lentivirus to transduce primary T cells. If anyone has any experience in this area I will really appreciate your suggestions. Is it difficult to transduce T cells with lentivirus? Do you have to activate the T cells for this?
Thanks,
Meenu

Meenu Pillai, Ph.D
Postdoctoral fellow,
St Jude Children's Research Hospital
Memphis, TN

________________________________
From: Vinko Tosevski [mailto:vinko.tosevski@medri.hr]
Sent: Wednesday, March 12, 2008 5:48 AM
To: cyto-inbox
Subject: FACSAria Accudrop issue. .

Hi,

Recently, we began experiencing some difficulties with setting up drop delay via Accudrop beads on our Aria. Before, the bead event rate of 4000-5000 was sufficient to see all the beads with optical filter activated (100% in center stream). Now, at that event rate, you don't see any beads - when you activate optical filter at that event rate, in couple of seconds the percentage in center stream decreases to 0%. You have to increase the pressure do that event rate reaches 10000-11000 in order to 100% of beads in center stream.

Is it dirty detector? Dirty optical filter? If yes, how can I clean it? Because, all the "windows" of the sort chamber are clean and it seems that I can't access anything else without disassembling the machine, which I don't want to do... Any advice? Am I overlooking something obvious?

Regards,
Vinko

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Vinko Tosevski, MMolBiol
Research and Teaching Assistant
Department of Physiology and Immunology
Rijeka Medical School

B.Branchetta 20
HR-51000 Rijeka
Croatia

tel: +385 51 651192
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