I would suggest the lightning link kit from Innova biosciences. The 100ug LL-PE goes for approx 50ug of antibody in approx 50ul. I actually exchange the antibodies into PBS prior to conjugation using a nanosep tube and remove potential clumps afterwards. The conjugates I did with polyclonals about 1 year ago are still working fine. As direct labelled antibodies they are easy to use and you do not have to worry about the off rates or cross reactivity and NSB of secondary antibodies. They also do a 1mg PE kit which would work for half a mg of antibody. You can also get the Lynx kit from AbD serotec. Have fun Gerhard Nebe-von-Caron Research Scientist and Biomedical Engineer SPD-Spark Swiss Precision Diagnostics Priory Business Park Bedford, MK44 3UP, UK Tel +44(0)1234-835474 Fax +44(0)1234-835002 mailto:g.nebe-von-caron@spdspark.com ________________________________ From: Benoit Labarthe [mailto:benoitlabarthe@gmail.com] Sent: 04 March 2008 02:51 To: cyto-inbox Subject: Ab-fluorochrome conjugation Hello flowers We are looking for a good method to conjugate low quantities of antibody (around 100µg). Do you have any advices/experiences concerning the bulk fast conjugation methods for around 1 mg of antibody compared to low quantity conjugation like the Zenon technology. Thank you for your help! benoit labarthe -- Benoît Labarthe, PharmD Institut de Cardiologie de Montréal Montreal Heart Institute Centre de Recherche, Laboratoire du Dr.Théroux 5000 Bélanger Est Montréal Québec Canada H1T 1C8 Tel (514)-376 3330 ext 3017 Fax (514)- 376 1076Received on Sat Mar 8 15:38:00 2008
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